Pectate Lyase (PL) Assay Kit (CWMAP-K021M)

Cat.No.

CWMAP-K021M

Method

Microplate-based method

Product size

96T

Product description

Pectate lyase (transeliminase, EC 4.2.2.10) is a type of pectinase that breaks down pectin polymers through trans-elimination. It is mainly derived from microorganisms and can increase the juice yield of fruits, and has potential application value in reducing environmental pollution. Pectate lyase (PL) acts on the α-1,4 glycosidic bonds in pectin to produce unsaturated oligogalacturonic acids with unsaturated bonds between C4 and C5 at the reducing end, which have a characteristic absorption peak at 235nm.

Additional Materials and Equipments Required

Microplate reader, 96-well plate (UV plate), balance, low-temperature centrifuge, temperature-controlled water bath, mortar.