Phosphofructokinase: Fructose-6-phosphate-1-phosphotransferase (PFP) Assay Kit (PHSAP-K011S)

Cat.No.

PHSAP-K011S

Method

Spectrophotometry

Product size

48T

Product description

Phosphofructokinase: fructose-6-phosphate-1-phosphotransferase (PFP, EC2.7.1.90) is a cytosolic enzyme widely present in plant tissues, catalyzing the reversible conversion between fructose-6-phosphate and fructose-1,6-bisphosphate, playing an important role in photosynthetic carbon metabolism. PFP catalyzes the conversion of fructose-6-phosphate to fructose-1,6-bisphosphate, which is then converted to dihydroxyacetone phosphate under the action of aldolase and triose phosphate isomerase, and further catalyzed by 3-phosphoglycerate dehydrogenase, causing a decrease in the absorbance of NADH at 340 nm, thereby reflecting the activity level of PFP enzyme.

Additional Materials and Equipments Required

UV-Vis spectrophotometer, 1mL quartz cuvette (path length 1cm), low-temperature centrifuge, pipettes, pipette, mortar, ice, and distilled water.