Phospholipid (PLIP) Assay Kit (Oxidase Method) (MBMQP-K027M)

Cat.No.

MBMQP-K027M

Method

Microplate-based method

Product size

48T/96T

Note

Provide microplate

Product description

Phospholipids are hydrolyzed by phospholipase D to form choline and phosphatidic acid, which is then oxidized by choline oxidase to form hydrogen peroxide. Hydrogen peroxide reacts with 4-aminophenazone and DAOS to form a blue dye. This dye has the maximum absorption peak at 600nm, and the absorption intensity is proportional to the phospholipid content in the sample.

Additional Materials and Equipments Required

Microplate reader, 96-well plate, pipette, centrifuge, distilled water.