Phosphotransacetylase (PTA) Activity Assay Kit (TACAP-K018M)

Cat.No.

TACAP-K018M

Method

Microplate-based method

Product size

48T

Note

Provide microplate

Product description

Phosphotransacetylase (PTA, EC 2.3.1.8) is one of the key enzymes related to acetate metabolism. PTA catalyzes the reaction between coenzyme A and acetyl phosphate to produce acetyl-CoA and inorganic phosphate. The increase in inorganic phosphate is measured by the ammonium molybdate method to determine the activity of PTA. Reaction: CoA + acetyl phosphate ⇌ acetyl-CoA + phosphate

Additional Materials and Equipments Required

Microplate reader, 96-well plate, desktop centrifuge, pipette, mortar and pestle, ice, and distilled water.