Protein Content Assay Kit (Biuret Method) (NMSQP-K027S)

Cat.No.

NMSQP-K027S

Method

Spectrophotometry

Product size

48T/96T

Product description

In an alkaline solution, compounds with two or more amide groups (-CO-NH2) or similar groups react with copper(II) ions to form a purple complex, known as the biuret reaction. Protein molecules, containing numerous peptide bonds (-CONH-), undergo the biuret reaction, and the color intensity is proportional to the protein content within a certain concentration range, with a maximum absorption wavelength of 540nm upon spectroscopic scanning.

Additional Materials and Equipments Required

Visible spectrophotometer, 1mL glass cuvettes (optical path 1cm), desktop centrifuge, temperature-controlled water bath, pipettes.