Recombinant Rhesus NKG2D Protein(His tag)

Recombinant Rhesus NKG2D Protein(His tag) (RI0273NL)

Recombinant Rhesus NKG2D Protein (Q9MZJ7) (Phe78-Val216) with a polyhistide tag at the N-terminus was expressed in Insect cells.

Size Price Qty
50ug $390.00

PRODUCT INFORMATION

Cat.No.
RI0273NL
Synonyms
KLRK1; NKG2D; CD314
Species
Rhesus
Accession
Source
Insect cells
Tag
His
Predicted N Terminal
His 20
Form
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0, 5 % trehalose and 5 % mannitol.
Bio-activity
Immobilized Rhesus His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human ULBP1-Fch, The EC50 of human ULBP1-Fch is 0.12-0.28 μg/ml. Immobilized Rhesus His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human MICB-Fch, The EC50 of human MICB-Fch is 33.8-79 ng/ml.
Molecular Mass
Recombinant Rhesus NKG2D consists of 155 amino acids and has a calculated molecular mass of 18.3 kDa.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 95 % by SDS-PAGE.

Usage Guide

Storage
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.
Reconstitution
Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.
Warning
For research use only!

BACKGROUND

Background
Function as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8+ T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins (By similarity).

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