Recombinant Rhesus NKG2D Protein(His tag) (RI0273NL)

Cat.No.

RI0273NL

Synonyms

KLRK1; NKG2D; CD314

Species

Rhesus

Accession

NKG2D

Source

Insect cells

Tag

His

Predicted N Terminal

His 20

Form

Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0, 5 % trehalose and 5 % mannitol.

Bio-activity

Immobilized Rhesus His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human ULBP1-Fch, The EC50 of human ULBP1-Fch is 0.12-0.28 μg/ml. Immobilized Rhesus His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human MICB-Fch, The EC50 of human MICB-Fch is 33.8-79 ng/ml.

Molecular Mass

Recombinant Rhesus NKG2D consists of 155 amino acids and has a calculated molecular mass of 18.3 kDa.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 95 % by SDS-PAGE.

Background

Function as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8+ T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins (By similarity).