Recombinant Rat Cst3 Protein (NP_036969) (21-140aa) with C-terminus His tag was expressed in Insect cells.
1. Kim, J-T; Lee, S-J; Kang, M. A.; et al. Cystatin SN neutralizes the inhibitory effect of cystatin C on cathepsin B activity, CELL DEATH & DISEASE, 4, e974.
"In this study, we showed that CST1 was highly expressed in colon tumor tissues, compared with nontumor regions. Increased cell proliferation and invasiveness were observed in HCT116 cell lines stably transfected with CST1 cDNA (HCT116-CST1) but not in CST3-transfected cells. We also demonstrated that CST1-overexpressing cell lines exhibited increased tumor growth as well as metastasis in a xenograft nude mouse model. Interestingly, CST1 interacted with cystatin C (CST3), a potent cathepsin B (CTSB) inhibitor, with a higher affinity than the interaction between CST3 and CTSB in the extracellular space of HCT116 cells."