Serum Copper (Cu) Assay Kit (MBMQC-K050M)

Cat.No.

MBMQC-K050M

Method

Microplate-based method

Product size

96T

Note

Provide microplate

Product description

Under acidic conditions, copper is dissociated from ceruloplasmin and albumin. Ascorbic acid (reduced form) reduces the dissociated copper(II) ions to copper(I) ions, which then form a blue complex with the chromogen 3,5-DiBr-PAESA. The absorbance of the blue copper complex at 600nm is measured to calculate the concentration of serum copper.

Additional Materials and Equipments Required

Microplate reader, 96-well plate, pipette, centrifuge, distilled water.