Product description
TBA is metabolized by the liver, and elevated serum concentrations reflect liver parenchymal damage. Therefore, TBA measurement is valuable for monitoring chronic liver disease. Bile acids are specifically oxidized by 3α-hydroxysteroid dehydrogenase (3α-HSD) and oxidized β-nicotinamide adenine dinucleotide thioester (Thio-NAD) to form 3-keto steroids and reduced β-nicotinamide adenine dinucleotide thioester (Thio-NADH). The formed 3-keto steroids are further regenerated into bile acids and oxidized β-nicotinamide adenine dinucleotide (NAD) in the presence of 3α-HSD and Thio-NADH. The described recycling amplification increases the sensitivity of detection. The absorbance change of reduced β-nicotinamide adenine dinucleotide thioester (Thio-NADH) at 405nm over a unit of time is measured to determine the content of bile acids.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, pipette, centrifuge, distilled water.