Total Bile Acids (TBA) Assay Kit (Enzymatic Recycling Colorimetric Method) (MBMQT-K054M)

Cat.No.

MBMQT-K054M

Method

Microplate-based method

Product size

48T/96T

Note

Provide microplate

Product description

TBA is metabolized by the liver, and elevated serum concentrations reflect liver parenchymal damage. Therefore, TBA measurement is valuable for monitoring chronic liver disease. Bile acids are specifically oxidized by 3α-hydroxysteroid dehydrogenase (3α-HSD) and oxidized β-nicotinamide adenine dinucleotide thioester (Thio-NAD) to form 3-keto steroids and reduced β-nicotinamide adenine dinucleotide thioester (Thio-NADH). The formed 3-keto steroids are further regenerated into bile acids and oxidized β-nicotinamide adenine dinucleotide (NAD) in the presence of 3α-HSD and Thio-NADH. The described recycling amplification increases the sensitivity of detection. The absorbance change of reduced β-nicotinamide adenine dinucleotide thioester (Thio-NADH) at 405nm over a unit of time is measured to determine the content of bile acids.

Additional Materials and Equipments Required

Microplate reader, 96-well plate, pipette, centrifuge, distilled water.