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Trypsin (EC 3.4.4.4) is a type of protease that can catalyze the hydrolysis of amide bonds. It is an important digestive enzyme. Trypsin catalyzes the hydrolysis of N-benzoyl-DL-arginine-p-nitroanilide hydrochloride (BAPNA) to produce p-nitroaniline, which has a maximum absorption peak at 405nm. The activity of trypsin can be determined by measuring the rate of increase in absorbance.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (optical path 1cm), desktop centrifuge, pipette, balance, mortar and pestle, ice, and distilled water.
