3-Phosphoglycerate Kinase (PGK) Activity Assay Kit (GLYAP-K004M)

Cat.No.

GLYAP-K004M

Method

Microplate-based method

Product size

96T

Note

Provide microplate

Product description

3-Phosphoglycerate kinase (PGK) is a key enzyme in glycolysis, widely present in animals, plants, and microorganisms. It catalyzes the reaction of 3-phosphoglycerate and ATP to produce 1,3-bisphosphoglycerate, which then produces 3-phosphoglyceraldehyde and NAD+ under the action of 3-phosphoglyceraldehyde dehydrogenase and NADH. By measuring the decrease of NADH, the activity of PGK can be obtained.

Additional Materials and Equipments Required

Microplate reader, 96-well plate, pipette, balance, shaker, low-temperature centrifuge, mortar and pestle.