3-Phosphoglycerate Oxidase (GPO) Activity Assay Kit (FASAP-K044M)

Cat.No.

FASAP-K044M

Method

Microplate-based method

Product size

96T

Note

Provide microplate

Product description

3-Phosphoglycerate (G3P) is oxidized by 3-phosphoglycerate oxidase (GPO) to produce hydrogen peroxide (H2O2), which reacts with 4-aminoantipyrine to form a red quinone compound with a characteristic absorption peak at 510nm. By detecting the change in absorbance at 510nm, the activity of 3-phosphoglycerate oxidase (GPO) can be determined.

Additional Materials and Equipments Required

Microplate reader, 96-well plate, pipette, balance, low-temperature centrifuge.