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• Protein Biotinylation Service • Custom Lysate Preparation Service • Immunogenicity Test Service > Anti-Drug Antibody (ADA) Evaluation Service > Neutralizing Antibody (NAb) Test Service • Anti-Drug Antibody (ADA) Evaluation Service • Neutralizing Antibody (NAb) Test Service • Thanksgiving Day Sale • Enzyme Target & Activator & Inhibitor Screening Service • GPCR Screening Service • Ion Channel Screening Service • Bio-Layer Interferometry (BLI) • Electrophoretic Mobility Shift Assay (EMSA) Service • Transcription Factor Analysis > Electrophoretic Mobility Shift Assay (EMSA) Service > Chromatin Immunoprecipitation (ChIP) Assay Service > DNase I Footprinting Assay Service • Transporter Screening Service • Chromatin Immunoprecipitation (ChIP) Assay Service • Enzyme Activity Assay Service • DNase I Footprinting Assay Service • Co-Immunoprecipitation (Co-IP) • Surface Plasmon Resonance (SPR) Service • Pull Down Assay • Protein Mutant Library Construction • C1 Expression System • Protein Conjugation Service on Beads • Bacteria Expression • Yeast Expression • Mammalian Cell Expression • Insect Cell Expression • Animal-Free Expression • Cell-Free Protein Expression • Large-Scale Protein Production • PEGylation Products and Services > Custom PEGylation Services > PEGylation Products • Basic Protein Characterization • Protein Labeling • Stable Cell Line Construction for Protein Expression • Protein Mutagenesis • Protein Interaction Analysis Services > Bio-Layer Interferometry (BLI) > Co-Immunoprecipitation (Co-IP) > Surface Plasmon Resonance (SPR) Service > Pull Down Assay > Yeast Two-Hybrid Screening > Membrane-Based Yeast Two-Hybrid Screening > CLIP-Seq Service > Protein Binding Site Mapping > Isothermal Titration Calorimetry > Membrane Protein Binding Screening and Profiling • Yeast Two-Hybrid Screening • Membrane-Based Yeast Two-Hybrid Screening • Recombinant Protein or Antibody Production • Protein Expression Systems > Nicotiana Tabacum Expression System > Bacterial Expression Systems(E. coli / Bacillus) > Yeast Expression Systems(P pastoris / S cerevisiae) > Baculovirus-insect Cell Expression Systems > Mammalian Expression Systems(CHO / 293) • PEGylation Services • Stable Cell Line Construction for Protein Expression • Process Development • cGMP Manufacturing • QC and QA • Bacterial Manufacturing • CLIP-Seq Service • Protein Binding Site Mapping • Quantum Dot Labeling • Protein Expression > Custom Lysate Preparation Service > C1 Expression System > Protein Co-Expression Service in E. coli System > Protein Co-Expression Service in Mammalian Cells > Trimeric Protein Production Service > Lettuce Transient Expression System • Protein Co-Expression Service in E. coli System • Protein Co-Expression Service in Mammalian Cells • NovEgg Expression System • ADME/Tox Prediction • Pharmacophore Modeling • Screening and Profiling Services > Enzyme Target & Activator & Inhibitor Screening Service > GPCR Screening Service > Ion Channel Screening Service > Transporter Screening Service • Trimeric Protein Production Service • Incorporation of Bioorthogonal Handles > Incorporation of Unnatural Amino Acids > Incorporation of Unnatural Sugars > Direct Chemical Modification • Incorporation of Unnatural Amino Acids • Incorporation of Unnatural Sugars • Protein/Peptide Crosslinking > Enzymatic Crosslinking Services > Photocrosslinking Services > Chemical Crosslinking Services • Enzymatic Crosslinking Services • Photocrosslinking Services • Chemical Crosslinking Services • Direct Chemical Modification • Protein-Fluorophore Bioconjugation • Custom PEGylation Services • PEGylation Products • Target Protein Degradation - Proteolysis Targeted Chimera (PROTAC) • Induced Protein Degradation > Target Protein Degradation - Proteolysis Targeted Chimera (PROTAC) > Hydrophobic Tag Services > Destabilization Domains (DDs) • Hydrophobic Tag Services • Destabilization Domains (DDs) • Drug Development • Protein Analysis > Cell-based Kinase Assays > Protein Aggregation Analysis • Protein Production > Anaerobic Fermentation • Anaerobic Fermentation > Lactic Acid Bacteria Fermentation • Cell-based Kinase Assays • Isothermal Titration Calorimetry • Lactic Acid Bacteria Fermentation • Protein Aggregation Analysis Nicotiana Tabacum Expression System • Lettuce Transient Expression System • Membrane Protein Binding Screening and Profiling

CONTACT US

Tel: 1-631-448-8149
Fax: 1-631-938-8127

Bacterial Expression Systems(E. coli / Bacillus)

Over the years, Profacgen has developed BacTECTM and FoldEZTM technologies to tackle hard-to-express and hard-to-dissolve proteins. With BacTECTM technology, the expression level can be greatly improved for almost all of the proteins. In addition, over 98% of the inclusion bodies can be refolded and solubilized by our proprietary FoldEZTM protein refolding technology.

Our OxiCytoTM competent E.coli strains are excellent options for expressing proteins having complex disulfide bonds.


The first choice for the expression of recombinant proteins is typically E. coli. Production of proteins in this bacterial strain is well-established, fast, simple and usually provides high yields.

Newly-built facilities supporting a vast array of state-of-the-art equipment are leveraged by our experienced PhD-level scientists to deliver proteins expressed via microbial systems quickly and economically.

One of the most popular technologies is BacTECTM system. Profacgen's patented BacTECTM system is capable of achieving soluble expression levels well over 20 g/L. While the technology has shown it can express many types of proteins, it has established a track record for producing high-quality, correctly folded antibody fragments (including Fabs and scFv). Our FoldEZTM protein refolding technology is designed to determine the optimal refolding conditions by evaluation a series of buffers. We provide refolded protein at purity of 95% or greater.

Exclusively from Profacgen, OxiCytoTM competent E.coli strains are engineered to enable the folding of recombinantly expressed proteins in vivo. The expression of the isomerase, DsbC, within the OxiCyto cytoplasm enhances the correct formation of disulfide bonds in proteins that require them for biological activity

Our Bacillus subtilis system is an ideal option for the expression of monomeric protein products at expression levels up to 20g/L. This high yield, combined with our patented fermentation protocols allow for fast process development and scale-up to support early clinical testing.

CONTACT US
Email: info@profacgen.com
Tel: 1-631-448-8149
Fax: 1-631-938-8127
Address: 45-1 Ramsey Road, Shirley, NY 11967, USA