Chloroplast 3-Phosphoglycerate Kinase (PGK) Activity Assay Kit (PHSAC-K019S)

Cat.No.

PHSAC-K019S

Method

Spectrophotometry

Product size

48T

Product description

Chloroplast 3-phosphoglycerate kinase (PGK) is a key enzyme in the Calvin cycle. It catalyzes the reaction of 3-phosphoglycerate and ATP to produce 1,3-bisphosphoglycerate, which is then acted upon by 3-phosphoglycerate dehydrogenase and NADH to produce 3-phosphoglyceraldehyde and NAD+. By measuring the decrease in NADH, the activity of 3-phosphoglycerate kinase can be determined.

Additional Materials and Equipments Required

UV-Vis spectrophotometer, 1mL quartz cuvette (path length 1cm), pipettes, balance, shaker, low-temperature centrifuge, mortar.