Cinnamate 4-Hydroxylase (C4H) Assay Kit (PMSAC-K017S)

Cat.No.

PMSAC-K017S

Method

Spectrophotometry

Product size

24T/48T

Product description

Cinnamate-4-hydroxylase (C4H, EC 1.14.13.11) is a key enzyme in the phenylpropanoid metabolic pathway. It can effectively regulate the synthesis of secondary metabolites such as flavonoid compounds, and its activity can be used as a physiological indicator of plant resistance. The principle of this product test is to use cinnamic acid as a substrate, under the optimal conditions of enzymatic reaction, cinnamate-4-hydroxylase catalyzes the substrate to produce 4-hydroxy-cis-cinnamic acid, which has the maximum absorption peak at 340nm under the optimal pH value, and thus the enzyme activity can be calculated.

Additional Materials and Equipments Required

UV spectrophotometer, 1mL quartz cuvette (optical path 1cm), tabletop centrifuge, pipette, mortar, ice, and distilled water.