D-Mannose-6-phosphate (M6P) Content Assay Kit (CASQS-K060S)

Cat.No.

CASQS-K060S

Method

Spectrophotometry

Product size

48T

Product description

This kit provides a quantitative, rapid, simple, and sensitive method for detecting D-mannose-6-phosphate (M6P) content. D-mannose-6-phosphate is converted into fructose-6-phosphate under the action of phosphomannose isomerase, and fructose-6-phosphate, under the action of an enzyme complex including phosphoglucose isomerase and glucose-6-phosphate dehydrogenase, converts NADP+ into NADPH. By detecting the increase of NADPH at 340nm, the content of D-mannose-6-phosphate (M6P) is calculated.

Additional Materials and Equipments Required

UV spectrophotometer, 1mL quartz cuvette (light path 1cm), balance, pipette, mortar and pestle, centrifuge, distilled water.