Endo-β-1,4-Glucanase/Cellobiohydrolase (CBH) Assay Kit - PNP Method (CASAC-K014M)

Cat.No.

CASAC-K014M

Method

Microplate-based method

Product size

48T/96T

Note

Provide microplate

Product description

Endo-β-1,4-glucanase, also known as cellobiohydrolase (CBH) (EC 3.2.1.91), is found in bacteria, fungi, and animals and is one of the components of the cellulase system. This enzyme catalyzes the hydrolysis of 4-nitrophenyl-β-D-cellobioside to produce p-nitrophenol (PNP), which has a maximum absorption peak at 405nm. The enzyme activity of endo-β-1,4-glucanase can be calculated accordingly.

Additional Materials and Equipments Required

Microplate reader, 96-well plate, pipette, balance, low-temperature centrifuge.