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Antioxidants can reduce Fe3+-TPTZ to blue Fe2+-TPTZ, and the ferric reducing ability of the sample can be obtained by measuring the blue Fe2+-TPTZ at 590nm. The higher the absorbance value, the stronger the reducing ability of the sample.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (light path 1cm), thermostatic water bath, low-temperature centrifuge, pipette, mortar, and distilled water.
