Product description
Glycerol, stored in fat cells, is one of the final products of triglyceride metabolism. In production and daily life, glycerol can be used as a solvent, lubricant, pharmaceutical, and sweetener. Glycerol is catalyzed by glycerol kinase (GK) to form glycerol-1-phosphate (G-1-P). G-1-P is oxidized by glycerol phosphate oxidase (GPO) to generate hydrogen peroxide (H2O2), which reacts with 4-aminoantipyrine to form a red quinone compound with a characteristic absorption peak at 510nm. By measuring the absorbance at 510nm, the glycerol content can be determined.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (optical path 1cm), desktop centrifuge, pipette, mortar, and distilled water.