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The glyoxalase system is the primary pathway for the removal of methylglyoxal (MG), and glyoxalase I (Gly I, EC 4.4.1.5) is a cytosolic enzyme that makes up the glyoxalase system. Glyoxalase I catalyzes the formation of S-D-lactoylglutathione (SLG) from methylglyoxal (MG) and reduced glutathione, which has a characteristic absorption peak at 240nm. By measuring the increase in absorbance at 240nm, the activity of glyoxalase I (Gly I) can be calculated.
Additional Materials and Equipments Required
Microplate reader (wavelength set to 240nm), 96-well UV plate, pipette, mortar, distilled water
