Hexokinase (HK) Assay Kit (GLYAH-K020M)

Cat.No.

GLYAH-K020M

Method

Microplate-based method

Product size

96T

Note

Provide microplate

Product description

Hexokinase (HK, EC 2.7.1.1) is widely present in animals, plants, microorganisms, and cultured cells and is one of the rate-limiting enzymes of the glycolytic pathway. HK phosphorylates glucose to produce glucose-6-phosphate, which further reacts with glucose-6-phosphate dehydrogenase and NADP, measuring the increase in NADPH absorption at 340 nm to calculate the activity of hexokinase.

Additional Materials and Equipments Required

Microplate reader, 96-well plate, temperature-controlled water bath, desktop centrifuge, pipette, mortar and pestle, ice, and distilled water.