Product description
Hydroxyproline (4-hydroxyproline, Hyp) is a non-essential amino acid and a major component of collagen tissue, found only in collagen and elastin in mammals but in many proteins in plants. Many diseases in animals are accompanied by changes in collagen metabolism, leading to changes in the content of hydroxyproline in blood, urine, and tissues. Therefore, detecting HYP content is an important reference indicator for understanding related diseases. This kit uses acid hydrolysis to produce free HYP, which is further oxidized by chloramine T and reacts with p-dimethylaminobenzaldehyde to show a purple-red color. By detecting the absorbance of the colored substance at 560nm, the HYP content can be determined.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (optical path 1cm), pipette, balance, low-temperature centrifuge.