L-Galactonolactone Dehydrogenase (GalDH) Activity Assay Kit (ASGAD-K021S)

Cat.No.

ASGAD-K021S

Method

Spectrophotometry

Product size

24T

Product description

L-Galactonolactone dehydrogenase (EC 1.1.1.316, L-galactose dehydrogenase, GalDH) is one of the important enzymes in the synthesis of ascorbic acid (Vc) in plants. L-Galactonolactone dehydrogenase directly oxidizes L-galactose at the C1 position to produce L-galactono-1,4-lactone, a direct substrate for Vc synthesis, while reducing NAD+ to NADH. This assay kit provides a simple, sensitive, and rapid method of measurement: the NADH produced in the enzymatic process reacts with a specific color developer to produce a colored substance with a maximum absorption peak at 450nm. The GalDH enzyme activity can be calculated by detecting the increase rate at 450nm.

Additional Materials and Equipments Required

Visible spectrophotometer, 1mL glass cuvette (light path 1cm), pipette, balance, low-temperature centrifuge.