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Lactose is broken down into galactose and glucose under the action of β-galactosidase, and then galactose is decomposed under the action of a complex enzyme containing galactose dehydrogenase, which reduces NAD+ to NADH. By detecting the increase of NADH at 340nm, the content of lactose and galactose can be calculated separately.
Additional Materials and Equipments Required
UV spectrophotometer, 1mL quartz cuvette (light path 1cm), centrifuge, pipette, mortar and pestle, and distilled water.
