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Lactose is broken down into galactose and glucose under the action of β-galactosidase, and then galactose is decomposed under the action of a complex enzyme containing galactose dehydrogenase, which reduces NAD+ to NADH. By detecting the increase of NADH at 340nm, the content of lactose and galactose can be calculated separately.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, centrifuge, pipette, mortar and pestle, ice, and distilled water.
