Lipoxygenase (LOX) Assay Kit (FASAL-K003S)

Cat.No.

FASAL-K003S

Method

Spectrophotometry

Product size

48T

Product description

Lipoxygenase (LOX, EC 1.13.11.12) is widely present in animal and plant tissues and catalyzes the oxidation of unsaturated fatty acids, leading to membrane lipid peroxidation. It plays an important role in the growth, development, maturation, aging, and stress response processes of organisms. Lipoxygenase (LOX) catalyzes the oxidation of linoleic acid, and the product has a characteristic absorption peak at 234nm. By measuring the increase in absorbance at 234nm, the activity of LOX can be calculated.

Additional Materials and Equipments Required

UV-Vis spectrophotometer, 1mL quartz cuvette (optical path 1cm), low-temperature desktop centrifuge, pipette, mortar, ice, and distilled water.