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Methanol (methanol) is converted into formaldehyde under the action of alcohol oxidase, and then reacts with 2,4-pentanedione to develop color. The colored substance has a specific absorption peak at 412nm; the content of methanol can be calculated based on this.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (light path 1cm), water bath, pipette, mortar and pestle.
