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Mitochondria are important sites for cellular respiration metabolism, and the H+-ATPase located on the inner mitochondrial membrane is a key component of oxidative phosphorylation coupling. H+-ATPase catalyzes the hydrolysis of ATP to produce ADP and inorganic phosphate, and this kit determines the enzyme activity by measuring the amount of inorganic phosphate.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, water bath, desktop centrifuge, pipette, mortar and pestle, ice, and distilled water.