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Na+K+-ATPase is widely distributed in plants, animals, microorganisms, and cells, and can catalyze the hydrolysis of ATP to produce ADP and inorganic phosphate. The enzyme activity is determined by measuring the amount of inorganic phosphate.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (optical path 1cm), water bath, desktop centrifuge, pipette, mortar and pestle, ice, and distilled water.