Phosphoglucomutase (PGI) / Phosphohexose Isomerase (PHI) Assay Kit (GLYAP-K026M)

Cat.No.

GLYAP-K026M

Method

Microplate-based method

Product size

96T

Note

Provide microplate

Product description

Phosphoglucomutase (PGI, EC 5.3.1.9) catalyzes the interconversion of fructose-6-phosphate and glucose-6-phosphate, an important intracellular enzyme involved in the glycolytic pathway during respiration. PGI catalyzes fructose-6-phosphate to glucose-6-phosphate, which is then reduced to NADPH by glucose-6-phosphate dehydrogenase with NADP+. By measuring the increase at 340nm, the activity of PGI can be calculated.

Additional Materials and Equipments Required

Microplate reader, 96-well plate, desktop centrifuge, pipette, mortar and pestle, ice, and distilled water.