Pyruvate Assay Kit (2,4-Dinitrophenylhydrazine Colorimetric Method) (GLYQP-K011M)

Cat.No.

GLYQP-K011M

Method

Microplate-based method

Product size

96T

Note

Provide microplate

Product description

Pyruvate plays an important role in various biochemical pathways, can be converted into carbohydrates during gluconeogenesis, or converted into fatty acids through acetyl CoA. Pyruvate reacts with 2,4-dinitrophenylhydrazine to form 2,4-dinitrophenylhydrazone, which turns brown-red in an alkaline solution; the content of pyruvate can be determined by reading the absorbance at 520nm.

Additional Materials and Equipments Required

Microplate reader, 96-well plate, desktop centrifuge, pipette, mortar and pestle, ice, distilled water.