Product description
Pyruvate plays an important role in various biochemical pathways, can be converted into carbohydrates during gluconeogenesis, or converted into fatty acids through acetyl CoA. Lactate dehydrogenase (LDH) can convert pyruvate into lactate, simultaneously oxidizing NADH. The decrease of NADH at 340nm is used to calculate the content of pyruvate.
Additional Materials and Equipments Required
UV-Vis spectrophotometer, 1mL quartz cuvette (light path 1cm), desktop centrifuge, pipette, mortar and pestle, ice, distilled water.