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Recombinant Cynomolgus CD3D&CD3E Protein(Fc-His & Fc-Flag tag) (CH0279CL)

Recombinant Cynomolgus CD3D Protein (Q95LI8) extracellular domain (Met 1-Ala 105) with the flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; Recombinant Cynomolgus CD3E Protein (Q95LI5) extracellular domain (Met 1-Asp 117) with the C-terminal his-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the Cynomolgus CD3D&CD3E heterodimer was purified.
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Size:20ug
Price:$390.00
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PRODUCT INFORMATION
Cat.No.
CH0279CL
Synonyms
CD3D; CD3 delta; T3D; CD3-DELTA; CD3E; T3E
Species
Cynomolgus
Accession
Source
HEK293
Tag
Fc-His & Fc-Flag
Predicted N Terminal
Gln 22 & Phe 22
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.
Molecular Mass
Recombinant heterodimer of Cynomolgus CD3E&CD3D comprises 679 (346+333) amino acids and has a calculated molecular mass of 76.7 (39.1+ 37.6) KDa. The apparent molecular mass of cyno CD3E&CD3D heterodimer is approximately 52 & 43 KDa respectively in SDS-PAGE.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
(50.3+48.5) % by SDS-PAGE.

Usage Guide
Storage
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.
Reconstitution
Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.
Warning
For research use only!

BACKGROUND
Background
T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

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