PDGFB; Putative uncharacterized protein
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.
Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 5-25 ng/ml. Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus PDGFRB-His at 10 μg/ml (100 μl/well) can bind Cynomolgus S4-mFc3-PDGFB, The EC50 of Cynomolgus S4-mFc3-PDGFB is 3.6-7.4 ng/ml.
Recombinant Cynomolgus PDGFB is a disulfide-linked homodimer. The reduced monomer comprises 345 amino acids and has a calculated molecular mass of 38.9 KDa. The apparent molecular mass of it is approximately 43 and 32 KDa in SDS-PAGE.
< 1.0 EU per 1 microgram of protein (determined by LAL method).
> 95 % by SDS-PAGE.