Recombinant Human AGO1 Protein(His tag) (HI0182AL)

Cat.No.

HI0182AL

Synonyms

AGO1; Argonaute1; hAgo1; eIF-2C 1; eIF2C 1; EIF2C1

Accession

AGO1

Predicted N Terminal

His

Form

Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% Gly, 0.5 PMSF, 0.5mM EDTA, pH 8.0, 5 % trehalose and 5 % mannitol.

Molecular Mass

Recombinant Human AGO1 consists of 875 amino acids and predicts a molecular mass of 99.5 kDa as estimated in SDS-PAGE under reducing conditions.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 97 % by SDS-PAGE.

Storage

In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.

Reconstitution

Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.

Warning

For research use only!

Background

Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).

Tag

His

Species

Human

Source

Insect cells

Background

Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).