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Recombinant Human CD3E&CD3D Protein(His & Flag tag) (HH0036CL)

Recombinant Human CD3E Protein (P07766) (Asp 23 - Asp 126) with his-tag at the C-terminus and Human CD3D Protein (P04234) (Phe 22 - Ala 105) with flag-tag at the C-terminus was co-expressed in HEK293.

Datasheet

Size Price Qty
50ug $759.00
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PRODUCT INFORMATION

Cat.No.
HH0036CL
Synonyms
CD3E; CD3-epsilon; T3E; TCRE; CD3D;
Accession
CD3E & CD3D
Predicted N Terminal
Asp 23 (CD3E) & Phe 22 (CD3D)
Form
Lyophilized from sterile PBS, pH7.4, 10% Trehalose.
Molecular Mass
Recombinant Human CD3E&CD3D heterodimer protein is produced by co-expression of CD3E and CD3D, has a calculated MW of 16.4 kDa (CD3E) and 14.4 kDa (CD3D). The reducing heterodimer protein migrates as 20 kDa and 22-28 kDa due to glycosylation.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 95 % by SDS-PAGE.
Storage
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.
Reconstitution
Dissolve with double distilled water.
Warning
For research use only!
Background
The CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. Required for normal immune responses (PubMed:15546002, PubMed:8490660).
Tag
His & Flag
Species
Human
Source
HEK293

BACKGROUND

Background
The CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. Required for normal immune responses (PubMed:15546002, PubMed:8490660).
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