Recombinant Human CDH1 Protein(Fc tag) (HH0102CL)

Cat.No.

HH0102CL

Synonyms

CDH1; UVO; cadherin-1; CD324; E Cadherin; E-Cadherin; CDHE; ECAD; LCAM; Arc-1;

Accession

CDH1

Predicted N Terminal

Gln 23 & Asp 155

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.

Bio-activity

Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells.
When cells are added to E-Cad coated plates (5 μg/mL, 100 μL/well), approximately 33% will adhere specifically after 90 minutes at 37 ?C

Molecular Mass

Recombinant Human E-Cad/Fc is a disulfide-linked homodimer. The reduced monomer comprises 791 amino acids and has a predicted molecular mass of 87.1 kDa. The apparent molecular mass of the protein is approximately 116-126 kDa in SDS-PAGE under reducing conditions.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 90 % by SDS-PAGE.

Storage

In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.

Reconstitution

Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.

Warning

For research use only!

Background

Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.

Tag

Fc

Species

Human

Source

HEK293

Background

Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.