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0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Bio-activity
The biological activity of Human Decorin was determined by its ability to modulate collagen fibrillogenesis (Ge, G. et al., 2004, J. Biol. Chem. 279:41626). At 5 μg/ml, rhDecorin can significantly delay the rate of type I collagen fibrillogenesis. Each investigator should determine their own optimal working dilution for specific applications.
Molecular Mass
The predicted molecular weight of Recombinant Human DCN is Mr 38 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 40-42 kDa.
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Purity
> 95% as determined by SDS-PAGE
Storage
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
Reconstitute at 300 μg/mL in sterile PBS.
Warning
For research use only!
Species
Human
Source
Insect cell
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