Recombinant Human LDLR Protein(His tag) (HH0050LL)

Cat.No.

HH0050LL

Synonyms

LDLR; LDL receptor; FH; FHC; LDLCQ2;

Accession

LDLR

Predicted N Terminal

Ala22 or Asp193

Form

Lyophilized from sterile PBS, pH7.4, 10% Trehalose.

Bio-activity

Immobilized Human LDL R, His Tag at 10 μg/mL (100 μl/well) can bind Biotinylated Human (D374Y) PCSK9 with a linear range of 0.02-0.5 μg/mL. Immobilized Human LDL R, His Tag at 10 μg/mL (100 μl/well) can bind Biotinylated Human PCSK9 with a linear range of

Molecular Mass

Recombinant Human LDLR Protein is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 86 kDa. The reducing protein migrates as 95-110 kDa &125-140 kDa in SDS-PAGE due to glycosylation.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 90 % by SDS-PAGE.

Storage

In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.

Reconstitution

Dissolve with double distilled water.

Warning

For research use only!

Background

Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes, but not through a direct interaction with viral proteins (PubMed:10535997, PubMed:12615904). Acts as a receptor for vesicular stomatitis virus (PubMed:23589850). In case of HIV-1 infection, may function as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells (PubMed:11100124).

Tag

His

Species

Human

Source

HEK293

Background

Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes, but not through a direct interaction with viral proteins (PubMed:10535997, PubMed:12615904). Acts as a receptor for vesicular stomatitis virus (PubMed:23589850). In case of HIV-1 infection, may function as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells (PubMed:11100124).