Recombinant Human NGF Protein (HC0166NL)

Cat.No.

HC0166NL

Synonyms

NGF; NGFB; HSAN5; Beta-NGF

Accession

NGF

Predicted N Terminal

Ser 122

Form

Lyophilized from sterile 20mM NaAc, 150mM NaCl, pH 5.5, 5 % trehalose and 5 % mannitol.

Bio-activity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-2 ng/ml.

Molecular Mass

Recombinant Human β-NGF consists of 118 amino acids and has a predicted molecular mass of 13.2 kDa. β-NGF exists as a non-disulfide linked homodimer in solution.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 95 % by SDS-PAGE.

Storage

In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.

Reconstitution

Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.

Warning

For research use only!

Background

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).

Species

Human

Source

CHO

Background

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).