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Recombinant Human SMPD1 Protein(His tag) (HI0172SL)

Recombinant Human SMPD1 Protein (P17405) (Met 1-Cys 631) with a polyhistidine tag at the C-terminus was expressed in Insect cells.

Datasheet

Size Price Qty
10ug $429.00
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PRODUCT INFORMATION

Cat.No.
HI0172SL
Synonyms
SMPD1; ASM; NPD; ASMASE
Accession
SMPD1
Predicted N Terminal
His 62
Form
Supplied as sterile 50mM Tris, 100mM NaCl, pH 8.0, 0.1% OGP, 10% glycerol.
Bio-activity
Measured by its ability to cleave. 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HNPPC). The specific activity is>1,000 pmol/min/μg.
Molecular Mass
Recombinant Human SMPD1 consists of 518 amino acids and predicts a molecular mass of 65 kDa as estimated by SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 94 % by SDS-PAGE.
Storage
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.
Warning
For research use only!
Background
Converts sphingomyelin to ceramide (PubMed:1840600, PubMed:18815062). Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol.
Tag
His
Species
Human
Source
Insect cells

BACKGROUND

Background
Converts sphingomyelin to ceramide (PubMed:1840600, PubMed:18815062). Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol.
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