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Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its ability to inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.2-0.8 μg/ml in the presence of 0.25 ng/mL recombinant human
Molecular Mass
Recombinant Human TNFRII comprises 246 amino acids and has a calculated molecular mass of 26.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TNFRII is approximately 42-45 kDa due to glycosylation.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 97 % by SDS-PAGE.
Storage
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.
Reconstitution
Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.
Warning
For research use only!
Background
Involved in lymphocyte activation. Binds to HLA class-II antigens (By similarity).
Tag
His
Species
Human
Source
HEK293
BACKGROUND
Background
Involved in lymphocyte activation. Binds to HLA class-II antigens (By similarity).
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