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Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its ability to bind biotinylated recombinant human B2M in a functional ELISA. Measured by its ability to bind biotinylated recombinant human FCGRT+B2M in a functional ELISA. Measured by its ability to bind biotinylated recombinant human LCK in a functional ELISA.
Molecular Mass
Recombinant Mouse CD8a consists of 180 amino acids and has a calculated molecular mass of 20.3 kDa. As a result of glycosylation, Recombinant protein migRates as an approximately 35 kDa protein in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 95 % by SDS-PAGE.
Storage
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.
Reconstitution
Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.
Warning
For research use only!
Background
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Tag
His
Species
Mouse
Source
HEK293
BACKGROUND
Background
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
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