Recombinant Mouse Ces1g Protein (His tag) (MI0022CS)

Cat.No.

MI0022CS

Synonyms

Liver carboxylesterase 1; CES1G; Ces-1; Ces1; Ses-1

Form

Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.

Molecular Mass

61.9kDa (556aa)
50-70KDa (SDS–PAGE under reducing conditions.)

AA Sequence

ADPHPSLPPV VHTVHGKVLG KYVTLEGFSQ PVAVFLGVPF AKPPLGSLRF APPEPAEPWS FVKHTTSYPP LCYQNPEAAL RLAELFTNQRKIIPHKFSED CLYLNIYTPA DLTQNSRLPV MVWIHGGGLV IDGASTYDGV PLAVHENVVV VVIQYRLGIW GFFSTEDEHS RGNWGHLDQVAALHWVQDNI ANFGGNPGSV TIFGESAGGE SVSVLVLSPL AKNLFHRAIA QSSVIFNPCL FGRAARPLAK KIAALAGCKT TTSAAMVHCLRQKTEDELLE VSLKMKFGTV DFLGDPRESY PFLPTVIDGV LLPKAPEEIL AEKSFNTVPY MVGINKHEFG WIIPMFLDFP LSERKLDQKT
AASILWQAYP ILNISEKLIP AAIEKYLGGT EDPATMTDLF LDLIGDIMFG VPSVIVSRSH RDAGAPTYMY EYQYRPSFVS DDRPQELLGDHADELFSVWG APFLKEGASE EEINLSKMVM KFWANFARNG NPNGEGLPHW PEYDQKEGYL QIGVPAQAAH RLKDKEVDFW TELRAKETAERSSHREHVEL HHHHHH

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Purity

> 90% by SDS - PAGE

Storage

Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.

Concentration

0.5mg/ml (determined by Absorbance at 280nm)

Warning

For research use only!

Background

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. The expression and activity of this gene is age-related but independent of growth hormone level. This gene is clustered with Ces7 and Ces3 on chromosome 8.

References

Satoh T., et al. (2006) Chem Biol Interact. 162:195-211.
Fleming CD., et al. (2007) Biochemistry. 46:5063-5071.

Tag

His tag

Species

Mouse

Source

Insect cells

Background

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. The expression and activity of this gene is age-related but independent of growth hormone level. This gene is clustered with Ces7 and Ces3 on chromosome 8.

References

Satoh T., et al. (2006) Chem Biol Interact. 162:195-211.
Fleming CD., et al. (2007) Biochemistry. 46:5063-5071.