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The ED50, calculated by the dose-dependant proliferation of murine BALB 3T3 cells (measured by 3H-thymidine uptake) is < 1.0 ng/ml, corresponding to a specific activity of 1MU/mg.
Molecular Mass
7600 Dalton
Protein length
70 amino acids
AA Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Pro-Glu-Thr-Leu.
Purity
Greater than 98.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Storage
Lyophilized IGFI although stable at room temperature for 3 weeks, should be stored desiccated below -18 centigrade. Upon reconstitution IGF-1 should be stored at 4 centigrade between 2-7 days and for future use below -18 centigrade. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Reconstitution
It is recommended to reconstitute the lyophilized IGF1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Warning
For research use only!
Species
Mouse
Source
E. coli
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