Reduced Ascorbic Acid (AsA)/Vitamin C Assay Kit (ASGQT-K008S)

Cat.No.

ASGQT-K008S

Method

Spectrophotometry

Product size

48T

Product description

Ascorbic acid (AsA), also known as Vitamin C, is an important antioxidant that plays a regulatory role in redox metabolic reactions. The ratio of AsA to dehydroascorbic acid (DHA) (AsA/DHA) is another important indicator of cellular oxidative status. Reduced ascorbic acid (AsA) reduces ferric ions to ferrous ions, which then react with 4,7-Diphenyl-1,10-phenanthroline to form a red complex with a characteristic absorption peak at 534nm. The color intensity is proportional to the content of reduced ascorbic acid.

Additional Materials and Equipments Required

Visible spectrophotometer, 1mL glass cuvette (light path 1cm), mortar and pestle, ice, low-temperature centrifuge, anhydrous ethanol.