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Recombinant Human CNTF Protein (HE0009CS)

Recombinant Human CNTF Protein (NP_000605) (1-200aa) was expressed in E.coli.
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Size:20ug
Price:$195.00
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PRODUCT INFORMATION
Cat.No.
HE0009CS
Synonyms
CNTF; ciliary neurotrophic factor; HCNTF;
Species
Human
Accession
Source
E. coli
Form
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Bio-activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is less than or equal to 500 ng/ml.
Molecular Mass
22.9 kDa (200aa), confirmed by MALDI-TOF
AA Sequence
MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARLLEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Purity
> 90% by SDS - PAGE

Usage Guide
Storage
Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
Concentration
1 mg/ml (determined by Bradford assay)
Warning
For research use only!

BACKGROUND
Background
The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse.
References
Sendther M., et al. (1994) J Neurobiol. 25(11):1436-53.
Robert F., et al. (2007) J Biol Chem. 282(46):33421-33434.

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