CNTF; ciliary neurotrophic factor; HCNTF;
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is less than or equal to 500 ng/ml.
22.9 kDa (200aa), confirmed by MALDI-TOF
MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARLLEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM
< 1.0 EU per 1 microgram of protein (determined by LAL method)
> 90% by SDS - PAGE
The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse.
Sendther M., et al. (1994) J Neurobiol. 25(11):1436-53.
Robert F., et al. (2007) J Biol Chem. 282(46):33421-33434.