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Recombinant Human AGO1 Protein(His tag) (HI0182AL)

Recombinant Human AGO1 Protein (Q9UL18) (Met 1-Ala 857) with a polyhistidine tag at the N-terminus was expressed in Insect cells.

Datasheet

Size Price Qty
20ug $429.00
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PRODUCT INFORMATION

Cat.No.
HI0182AL
Synonyms
AGO1; Argonaute1; hAgo1; eIF-2C 1; eIF2C 1; EIF2C1
Species
Human
Accession
AGO1
Source
Insect cells
Tag
His
Predicted N Terminal
His
Form
Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% Gly, 0.5 PMSF, 0.5mM EDTA, pH 8.0, 5 % trehalose and 5 % mannitol.
Molecular Mass
Recombinant Human AGO1 consists of 875 amino acids and predicts a molecular mass of 99.5 kDa as estimated in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 97 % by SDS-PAGE.

BACKGROUND

Background
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).
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