Lyophilized from sterile 10mM sodium citrate, pH3.5.
Bio-activity
BMP-2 is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of MC3T3-E1 cells is less than 50 ng/ml, corresponding to a specific activity of 2.0 x 10^4 IU/mg.
Molecular Mass
Recombinant Human BMP2 Protein is a homodimeric, non-glycosylated, Polypeptide chain and has a molecular mass of approximately 26.018 kDa.
Endotoxin
< 0.01 EU per 1 microgram of protein (determined by LAL method).
Purity
> 95 % by SDS-PAGE.
BACKGROUND
Background
Induces cartilage and bone formation (PubMed:3201241). Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4 (PubMed:24362451).
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