Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its ability to bind human CD28 in a functional ELISA. Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells. When 5 x 10^4 cells/well are added to CD4-coated plates (2.5 μg
Molecular Mass
Recombinant Human CD4 (aa 1-208) consists of 194 amino acids and has a predicted molecular mass of 21.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCD4 is approximately 26 kDa.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 94 % by SDS-PAGE.
BACKGROUND
Background
Involved in lymphocyte activation. Binds to HLA class-II antigens.
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